Thrombin and ionophore A23187-induced dense granule secretion in storage pool deficient platelets: evidence for impaired nucleotide storage as the primary dense granule defect
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چکیده
منابع مشابه
Thrombin and ionophore A23187-induced dense granule secretion in storage pool deficient platelets: evidence for impaired nucleotide storage as the primary dense granule defect.
The secretion of the dense granule constituents ATP, ADP, calcium, pyrophosphate (PPi), and orthophosphate (Pi), and the release of magnesium induced by thrombin and the divalent cation ionophore A23187 have been quantitated directly in gel-filtered platelets from patients with storage pool deficiency (SPD). Both the contents and the maximal amounts of the dense granule constituents secretable ...
متن کاملThrombin and lonophore A23187-Induced Dense Granule Secretion in Storage Pool Deficient Platelets: Evidence for Impaired Nucleotide Storage as the Primary Dense Granule Defect
The secretion of the dense granule constituents ATP. ADP, calcium. pyrophosphate (PP,). and orthophosphate (PJ. and the release of magnesium induced by thrombin and the divalent cation ionophore A231 87 have been quantitated directly in gel-filtered platelets from patients with storage pool deficiency (SPD). Both the contents and the maximal amounts of the dense granule constituents secretable ...
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An antigen-capture sandwich enzyme-linked immunosorbent assay (ELISA) was developed for a novel protein granulophysin, a constituent of the platelet dense granule (DG) membrane and used to characterize patients with dense granule storage pool deficiency (delta-SPD). The assay uses two monoclonal antibodies against the protein, one of which is conjugated to peroxidase. Purified DGs, an enriched ...
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would have been missed. Further, ethnic differences in incidence trends have only been observed in childhoodALLand are not present in other childhood cancers (eg, childhood brain tumors), suggesting that changes in ethnic classification are unlikely to be solely responsible for trend differences. In utero exposure to factors such as tobacco are likely to have remained stable or decreased in pre...
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The clinical manifestations of platelet dense ( delta ) granule defects are easy bruising, as well as epistaxis and bleeding after delivery, tooth extractions and surgical procedures. The observed symptoms may be explained either by a decreased number of granules or by a defect in the uptake/release of granule contents. We have developed a method to study platelet dense granule storage and rele...
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ژورنال
عنوان ژورنال: Blood
سال: 1983
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v61.1.154.bloodjournal611154